Human ES Cell (hESC) Lines
(Unpublished materials posted on this page have not been peer-reviewed.)

With exception of the lines listed in the NIH Registry and a few stated exceptions, cell lines listed here are not eligible for NIH funding.

How to Conduct Non-Federal Stem Cell Research Without Violating the Federal Stem Cell Funding Prohibition. (PDF)
This article written by Robert J. Kenney, Jr., a partner with Hogan & Hartson LLP, and the director of the firm’s federal grants practice, discusses the NIH guidelines and how to implement them in a way that will minimize improper use of federal funds for ineligible hESC research.

Protocol for feeder and conditioned medium independent maintenance of hESC:
The paper is full text open access until end of March. You have to register for access. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. (Xu et la., Nature Methods 2:185)

NIH-funding approved lines:

• NIH Embryonic Stem Cell Registry
  

Published lines:

• Istanbul Memorial Hospital, Turkey
              7 cell lines
              Published: Reprod Biomed Online 10:617
  
  
• People's Republic of China
              2 lines derived from 3 day embryos
              Published: Hum. Reprod. online 9 June 2005
  
  
  
• University of California San Francisco
              2 lines derived on human feeder serum free
              Published: Fertil Steri 83:1517
  
  
• King's College London, UNITED KINGDOM
                  1 line, with cystic fibrosis mutation
                  Published: Reprod Biomed Online 10:390
  
  
• BresaGen, Athens, GA, USA
                 1 subline from BG01, available at ATCC (# SCRC-2002 ),                                   karyotype: XXY, +12, +17.
  
  
• Seoul National University, Seoul, Korea
                 3 lines, published: Stem Cells, 23: 211
  
  
• Valencia Stem Cell Bank, Valencia, Spain
                 2 lines, under animal-free conditions
                 Published: Fertil Steril 83:246.
  
  
• MizMedi Hospital, Seoul, Korea
                 3 lines, derived on human feeder cells
                 Published: Biol Reprod 72:42.
  
  
• Reproductive Genetics Institute Chicago , IL , USA
                18 lines derived from embryos with genetic disorders.
                Published: Reprod Biomed Online 10: 105    
  
                8 Morula-derived cell lines.
                Published: Reprod Biomed Online 9: 623 
                  
  
• University of Newcastle upon Tyne, United Kingdom
                1 line, published: Stem Cells 22:790
               
  
• Royan Institute, Teheran, Iran
                1 line, published: Differentiation 75:224
               
  
• Cellartis AB, Goteborg, Sweden
                6 lines, published: Stem Cells 22:367
                [15 lines: 2 NIH-approved, 9 eligible for EU funding, 1 clonal]   
               
  
• Harvard University, Harvard U.S.A.
                17 lines, published: NEJM 350:1353
  
  
• Maria Infertility Hospital Medical Institute, Seoul, Korea
                 9 lines, published: Hum Reprod 19:676
  
  
• Rambam Medical Center, Haifa, Israel
                 1 line, published: Hum Reprod 19:670
  
  
• Seoul National University, Seoul, Korea
                 1 NT-derived cell line
                  P ublished: Science 303:1669
  
  
• MizMedi Hospital, Seoul, Korea
                 2 lines, published: Biol Reprod 69:2007
  
  
• King’s College London, UNITED KINGDOM
                 1 line, published: Reprod Biomed Online 7:353
  
  
• Karolinska Institute, Stockholm, Sweden
                 2 lines, published: Hum Reprod 18:1404
  
  
• National University of Singapore, Kent Ridge, Singapore
                 1 line, under animal-free conditions
                  Published: Nat Biotechnol 20:933
  

Unpublished lines:

• Brno, Czeck Republic
                 7 lines non-NIH approved, additional information: Report (PDF)
  
  
• Technion Haifa, Israel
                  Additional information: 2 lines, ISSCR 2004, abstract #79
  
  
• Russian Academy of Science, Moscow, Russia
                 Additional information: 3 lines, ISSCR 2004, abstract #141
  
  
• University of Helsinki, Finland
                4 lines, poster with characteristics
  
  
• University of San Francisco, San Francisco, U.S.A.
                 Additional information: 8 lines derived on human feeder cells,
                 ISSCR 2004, abstract #113
  

Additional Information and Resources:

• Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to august 9, 2001. (Brimble et al., Stem Cells Dev 13:585)

• Human embryonic stem cell lines derived from discarded embryos. (Mitalipova et al., Stem Cells 21:521)

• Differentiation of human embryonic stem cells to dopaminergic neurons in serum-free suspension culture. (Schulz et al., Stem Cells 22:1218)
  
  
• Available human feeder cells for the maintenance of human embryonic stem cells. (Lee et al., Reproduction 128:727)
  
  
• Long-term culture of human embryonic stem cells in feeder-free conditions and gene expression analysis ( Rosler et al., Dev Dyn 229:259)
  
  
• Review: Propagation and maintenance of undifferentiated human embryonic stem cells (Bodnar et al., Stem Cells Dev 13:243)
  
  
• Characterization of NIH-elibible lines by the NIH Stem Cell Unit
  
  
• Characterization of Batch 1 of HSF-6 ES Cell
  
  
• Characterization of BG01 and BG02 (Stem Cells 22:292)
  
  
• ES Cell Line Sequencing Information (WA01)
  
  
• Unique Gene Expression Signatures of Independently-Derived
  Human Embryonic Stem Cell Lines (Lines HSF-1, HSF-6 and H9,
  by Abeyta et al., Hum Mol Genet 13:601)
  
  
• National Stem Cell Resource at  ATCC

Protocols:

• hESC methods manual from BresaGen. Contains protocolls on isolation of MEFs, freezing and thawing of MEFs and hESCs, preparation of conditioned medium, microdissection and karyotyping. NIH Web site hESC methods manual from BresaGen.

• Efficient Transfection of Embryonic and Adult Stem Cells. Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS, Verfaillie CM (2004). Stem Cells 22:531.

• Controlled, Scalable Embryonic Stem Cell Differentiation Culture. Dang SM, Gerecht-Nir S, Chen J, Itskovitz-Eldor J, Zandstra PW (2004).
  Stem Cells 22:275.

• Electroporation of Murine Embryonic Stem Cells: A Step-by-Step Guide
  Tompers DM, Labosky PA (2004). Stem Cells 22: 243.